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Volume 9, Number 6, 2004

Megakaryocytes morphology in idiopathic (primary) myelofibrosis

Iryna V. Byelinska, Iryna N. Kabachenko, Iryna S. Dyagil


Background: The cause of idiopathic myelofibrosis (IM) originating from a haemopoietic stem cells with development of secondary marrow
fibrosis is unknown. Increased production of platelet-derived growth factor by megakaryocytes causes amplified activity of fibroblasts. Thus,
an analysis of changes in megakaryocyte population affecting fibrous expansion of the haemopoietic base is relevant.
Methods: Analysis and calculation of megakaryocytogram were carried out using bone marrow smears during routine examination of 12 patients with IM and 12 individuals without haematological disorders.
Results: Hyperthrombocytemia prevailed in the peripheral blood of patients with IM. A significant increase was revealed in the content of megakaryoblasts (p<0.05), naked nuclei (p<0.01) both with diminished count of polychromatophilic megakaryocytes (p<0.05). Cells with picnotic nuclei surrounded with cytoplasm completely decomposed into platelets were distinguished as a separate population with a mean number of 21 ± 3.0%, out of which basophilic cells made up 9 ± 1.5% and polycromatophilic cells - 12 ± 1.9% (vs. 1 ± 0.4% in control)of the group investigated. Decrease in the megakaryocyte population with 4-8 nuclei lobules (p<0.001) at the expense of an increase in the megakaryocytes number with tight picnotic nuclei, naked nuclei and an increase in the number of 1-2 lobuled forms (p<0.05) were found. Contents of atypical micromegakaryocytes, giant hyperlobuled and dysplastic forms of megakaryocytes was increased.
Conclusion: The changes revealed characterise differentiation disorder in the process of megakaryocytopoiesis. Megakaryocytes are subject to accelerated ageing and enhanced destruction with simultaneous prominent platelet production.

Signature: Rep Pract Oncol Radiother, 2004; 9(6) : 223-228


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