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Volume 19, Number S1, 2014

Investigation of the bystander effect in CHO-K1 cells

Urszula Kaźmierczak, Dariusz Banaś, Janusz Braziewicz, Iwona Buraczewska, Joanna Czub, Marian Jaskóła, Łukasz Kaźmierczak, Andrzej Korman, Marcin Kruszewski, Anna Lankoff, Halina Lisowska, Marta Nesteruk, Zygmunt Szefliński, Maria Wojewód

Summary:

Aim

Investigation of the bystander effect in Chinese Hamster Ovary cells (CHO-K1) co-cultured with cells irradiated in the dose range of 0.1–4 Gy of high LET 12C ions and X-rays.

Background

The radiobiological effects of charged heavy particles on a cellular or molecular level are of fundamental importance in the field of biomedical applications, especially in hadron therapy and space radiation biology.

Materials and methods

A heavy ion 12C beam from the Heavy Ion Laboratory of the University of Warsaw (HIL) was used to irradiate CHO-K1 cells. Cells were seeded in Petri dishes specially designed for irradiation purposes. Immediately after irradiation, cells were transferred into transwell culture insert dishes to enable co-culture of irradiated and non-irradiated cells. Cells from the membrane and well shared the medium but could not touch each other. To study bystander effects, a clonogenic survival assay was performed.

Results

The survival fraction of cells co-cultured with cells irradiated with 12C ions and X-rays was not reduced.

Conclusions

The bystander effect was not observed in these studies.

Signature: Rep Pract Oncol Radiother, 2014; 19(S1) : 37-41


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Indexed in: EMBASE®, the Excerpta Medica database, the Elsevier BIOBASE (Current Awareness in Biological Sciences) and in the Index Copernicus.

http://www.sciencedirect.com/science/journal/15071367/19/2